Journal: Journal of Food and Drug Analysis

Article Title: Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants

doi: 10.38212/2224-6614.3354

Figure Lengend Snippet: The specificity assay of the species-specific primers in multi-single PCR reaction. (A) The specificity of IiPF-CR; (B) The specificity of BcPF2-CR; (C) The specificity of PtPF3-CR; (D) 18S rRNA internal control; Lane M, 100 bp DNA ladder marker; Lane 1, DNA of Isatis indigotica standard raw material (j-1-2-07); Lane 2, DNA of Baphicacanthus cusia standard raw material (L-3-3-02); Lane 3, DNA of Polygonum tinctorium standard raw material (j-2-1-01); Lane 4, DNA of the powder mixtures of I. indigotica, B. cusia and P. tinctorium (50 mg + 50 mg + 50 mg); Lane 5, DNA mixtures (50 ng/μL) by mixing the templates of I. indigotica (j-1-2-07), B. cusia (L-3-3-02) and P. tinctorium (j-2-1-01); Lane N, negative control.

Article Snippet: PCR products were analyzed using 2% agarose gel electrophoresis, stained with EZ-VisionTM Two Dye as Loading Buffer (AMRESCO, Solon, OH, USA) with the inclusion of a EZ-Vision 100 base pairs (bp) DNA ladder (AMRESCO, Solon, OH, USA) and visualized under ultraviolet (UV) illumination.

Techniques: Control, Marker, Raw Material, Negative Control

Journal: Journal of Food and Drug Analysis

Article Title: Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants

doi: 10.38212/2224-6614.3354

Figure Lengend Snippet: DNA gel electrophoresis of multiplex PCR. DNA gel analysis showing the effectiveness of the multiplex PCR of individual target species (controls) with serial dilutions DNA mixtures (50 ng, 20 ng, 10 ng, 1 ng, 0.1 ng and 0.01 ng per microliter) by mixing the templates of Isatis indigotica (j-1-2-07), Baphicacanthus cusia (L-3-3-02) and Polygonum tinctorium (j-2-1-01). Lane M, 100 bp DNA ladder marker; Lane 1, DNA of I. indigotica standard raw material (j-1-2-07); Lane 2, DNA of B. cusia standard raw material (L-3-3-02); Lane 3, DNA of P. tinctorium standard raw material (j-2-1-01); Lane 4, DNA mixtures (50 ng/μL); Lane 5, DNA mixtures (20 ng/μL); Lane 6, DNA mixtures (10 ng/μL); Lane 7, DNA mixtures (1 ng/μL); Lane 8, DNA mixtures (0.1 ng/μL); Lane 9, DNA mixtures (0.01 ng/μL); Lane N, negative control.

Article Snippet: PCR products were analyzed using 2% agarose gel electrophoresis, stained with EZ-VisionTM Two Dye as Loading Buffer (AMRESCO, Solon, OH, USA) with the inclusion of a EZ-Vision 100 base pairs (bp) DNA ladder (AMRESCO, Solon, OH, USA) and visualized under ultraviolet (UV) illumination.

Techniques: DNA Gel Electrophoresis, Multiplex Assay, Marker, Raw Material, Negative Control

Journal: PLoS ONE

Article Title: TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

doi: 10.1371/journal.pone.0186568

Figure Lengend Snippet: 1 : DNA ladder. 2 : pET-BccI untreated. 3 : pET-BccI digested with BccI. 4 , 5 , 6 : pET-BccI digested with EcoRI, BamHI and HindIII, respectively.

Article Snippet: One percent agarose (Nippon Genetics) gels were used for the separation of the DNA bands and the Nippon Genetics 100 base pair (bp) DNA Ladder was used as a molecular weight indicator.

Techniques: